St. Jude Children’s Research Hospital has been recognized by FORTUNE magazine as one of the “100 Best Companies to Work For” for the second consecutive year. Last year was the hospital’s first appearance in the publication’s annual list. Employees again ranked pride in the hospital’s mission—finding cures, saving children—as one of the top reasons St. Jude is a great place to work.

“Our employees look at the work they do at St. Jude as much more than a job,” said Dr. William E. Evans, St. Jude director and CEO. “As we celebrate our 50th anniversary this year, we are reminded that one of the keys to our success has been attracting outstanding people and giving them a place to do their best work. Employees embrace the mission and espouse the culture of St. Jude, which is one of collaboration, compassion, innovation and quality in all we do.”

St. Jude strives to continually improve in all areas, including work environment for employees. Based on employee ideas and needs, the hospital has created initiatives that have enhanced communication between senior leadership and staff; enhanced the Wellness Program with a comprehensive approach to fitness and health; and fostered programs and policies intended to promote diversity.

To select businesses for the “100 Best Companies to Work For” list, FORTUNE partners with the Great Place to Work Institute to conduct the most extensive survey of employees done in corporate America. More than 280 companies participated in this year’s survey. Two-thirds of a company’s score is based on the results of the Institute’s Trust Index survey, which is sent to a random sample of employees at each company. The survey asks questions that assess their attitudes about management’s credibility, job satisfaction and camaraderie.

The other third of the scoring is based on the company’s responses to the institute’s culture audit, which includes detailed questions about pay and benefit programs and a series of open-ended questions about hiring practices, internal communications, training, recognition programs and diversity efforts. Any company that is at least five years old with more than 1,000 U.S. employees is eligible.

Ranked No. 39 on this year’s list, St. Jude was No. 80 in 2011.

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